FIG. 9.
Test of the importance of L-Sox5/Sox6 for Sox9 binding to cartilage enhancers. (A) ChIP in Sox5fl/fl Sox6fl/fl chondrocytes 48 h after infection with no adenovirus or AdeCre. Cartilage enhancers were detected by PCR of chromatin precipitated with antibodies as indicated. Binding of RNA polymerase II (Pol2) to the Gapdh promoter was used as positive control. (B) Quantitative ChIP assay of the same samples as in panel A, precipitated with IgG control (−) and Sox antibodies, as indicated. (C) Model for the mode of transactivation of Agc1 by the Sox trio. See text for details.