FIG. 2.

EGFrps directly and preferentially interact with PS. (A) Affinity of recombinant stabilin-2 proteins to PS. ELISA plates were coated with PS and then incubated with the indicated stabilin-2 proteins. After 1 h, proteins bound to the wells were quantified using anti-His antibody. (B) ELISA plates were coated with PS, PC, phosphatidylethanolamine (PE), or phosphatidylinositol (PI) and then incubated with the EGFrp protein (E3). After 1 h, proteins bound to the wells were quantified using anti-His antibody. The results are expressed as the means ± the SD from at least three experiments.