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. 2008 Jun 30;28(17):5209–5222. doi: 10.1128/MCB.00360-08

FIG. 8.

FIG. 8.

Partial impairment of cytokine expression in differentiated CD4+ and CD8+ T cells from the mixed ICR background Orai1−/− mice. T cells isolated from wild-type littermates (WT) or Orai1−/− mice (KO) were cultured under the indicated conditions for 6 days (see Materials and Methods). The cells were then restimulated with 10 nM PMA and 1 μM ionomycin (PMA/iono), with or without pretreatment with 1 μM cyclosporine A (CsA). Cyclosporine A was kept in the cultures after pretreatment. The percentage of cytokine-expressing cells is indicated in each quadrant. Data are representative of the results of at least three independent experiments. (A) Cells cultured under THN conditions and assessed for IL-2 and IFN-γ expression. (B) Cells cultured under TH1 conditions and assessed for IL-2 and IFN-γ expression. (C) Cells cultured under TH2 conditions and assessed for IL-10 and IL-4 expression. (D) Cytolytic T cells (CTLs) cultured in the presence of 100 U/ml human IL-2 were assessed for IFN-γ and granzyme B expression.