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. 2008 Jun 30;28(17):5494–5506. doi: 10.1128/MCB.00265-08

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FIG. 3. — Hsp90β knockdown partially restores sensitivity to cytochrome c in Ba/F3 cells expressing Tel-PDGFRβ (A to C) or FLT3/D835Y (D to F). (A and D) Ba/F3 cells expressing Tel-PDGFRβ (A) or FLT3/D835Y (D) were treated with Hsp90β-specific siRNA or control siRNA. Total cell lysates were immunoblotted for Hsp90β, Hsp90α, Tel-PDGFRβ, FLT3, and actin. (B and E) Caspase activity was assayed by measuring cleavage of DEVD-pNA following incubation of the cell lysates with 5 ng/μl cytochrome c and 1 mM dATP. (C and F) Immunoblotting was performed for caspase-9 and caspase-3 upon addition of various amounts of exogenous cytochrome c (Cyt c) to the lysates. Procaspase-9/procaspase-3 and cleaved caspase-9/caspase-3 are indicated by arrows and arrowheads, respectively. RNAi, RNA interference.