FIG. 2.
Ynr024w is the yeast homologue of the human exosome cofactor Mpp6. (A) The alignment shows the two conserved Mpp6 motifs from model eukaryotes, with consensus residues in bold. Start and end positions are indicated. K. lactis, Kluyveromyces lactis; E. gossypii, Eremothecium gossypii; D. hansenii, Debaryomyces hansenii; C. albicans, Candida albicans; A. nidulans, Aspergillus nidulans; D. discoideum, Dictyostelium discoideum; H. sapiens, Homo sapiens; D. melanogaster, Drosophila melanogaster; C. elegans, Caenorhabditis elegans; and A. thaliana, Arabidopsis thaliana. (B) Double-mutant strains carrying either the mpp6Δ PGAL-RRP47 or the rrp47Δ PGAL-MPP6 mutations are inhibited in growth 6 h after transfer to glucose medium. The wild-type (WT) strain and mutant strains carrying the PGAL-RRP47, PGAL-MPP6, mpp6Δ PGAL-RRP47, or rrp47Δ PGAL-MPP6 mutations were grown at 25°C in rich medium containing 2% galactose and then transferred for the time indicated on the x axis to rich medium containing 2% glucose. (C) Mpp6-GFP is restricted to the nucleus. In the merged image, Mpp6-GFP is shown in green and DAPI in blue. The nucleus is outlined with a dotted line.