TABLE 1.
Strains used in this study
| Strain | Genotypea | Reference or source |
|---|---|---|
| BY4741 | MATahis3D1 leu2D0 met15D0 ura3D0 | 9 |
| D658 | ade2 arg4 leu2-3,112 trp1-289 ura3-52 Csl4-TAP::KlURA3 | Euroscarf |
| YLM151 | As BY4741 but Mpp6-TAP::HIS | 18 |
| D270 | MATaade2 his3 leu2 trp1 ura3 | 57 |
| D271 | MATaade2 his3 leu2 trp1 ura3 | 57 |
| YLM140 | As D270 but mpp6D::NATR | This study |
| YLM142 | As D270 but HIS::GAL1-3HA-mpp6 | This study |
| YLM144 | As D270 but rrp47D::KANR | This study |
| YLM146 | As D270 but HIS::GAL1-3HA-rrp47 | This study |
| YLM148 | AS YLM140 but HIS::GAL1-3HA-rrp47 | This study |
| YLM150 | As YLM144 but HIS::GAL1-3HA-mpp6 | This study |
| YLM152 bob-GFP | As D270 but mpp6-eGFP::KANR | This study |
| YLM155 | As D270 but KANR::GAL1-3HA-rrp41 | This study |
| D342 | ura3-52 mtr3-1 | 26 |
| YLM156 | As D271 but rrp47D::NATR | This study |
| rna14.1 | MATaade2-1 his3-11 leu2-3,112 trp1-1 ura3-1 rna14.1 | 30 |
| YLM160 | As rna41.1 but rrp6D::KANR | This study |
| YLM161 | As rna14.1 but mpp6D::NATR | This study |
| prp2-1 | MATaura3-52 prp2-1 | 35 |
| YLM163 | As prp2-1 but rrp6D::KANR | This study |
| YLM165 | As prp2-1 but mpp6D::NATR | This study |
| BMA38a | MATaade2-1 his3 Δ200 leu2-3,112 trp13-1 ura3-1 can1-100 | 5 |
| YDC40 trf4Δ | As BMA38a but trf4Δ::KANRMX4 | 11a |
| YDC46 air1Δ/air2Δ | As BMA38a but air1Δ::KANRMX4 air2Δ::NATRMX6 | 11a |
| NRD1 | MATα ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 | YJC1163 45 |
| nrd1.102 | MATα ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 nrd1-102 | YJC1166 45 |
| nab3.11 | MATα ade2 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 nab3-11 | 12 |
a
3HA, triple-hemagglutinin tag; KlURA3, Kluveromyces lactis URA3.