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. 2008 Jun 30;28(17):5312–5327. doi: 10.1128/MCB.00128-08

FIG. 1.

FIG. 1.

KDM5b represses target genes through demethylation of 3meH3K4. (A) Change in 3meH3K4 after 24 h of transient transfection of mESCs with either Flag-EGFP or Flag-KDM5b as measured by Western blotting with either anti-3meH3K4 (Upstate Biotechnology) or anti-Pan H3 (Upstate Biotechnology). (B) qRT-PCR of KDM5b, Egr1, p27, and BMI1 (primers are listed in Table S1 in the supplemental material) after 24 h transfection of either the control (Flag-EGFP), Flag-KDM5b, or siKDM5b (IDTdna). (C, D, and E) qChIP analysis of 3meH3K4 binding or KDM5b binding to Egr1, BMI1, or p27 promoters (primers are listed in Table S2 in the supplemental material). (F) qChIP analysis of 3meH3K4 in Egr1 and p27 promoters after cells were treated with siKDM5b. *, P = 0.05; **, P < 0.01. All data are in triplicate except where noted.