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. 2008 Jun 30;28(17):5312–5327. doi: 10.1128/MCB.00128-08

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FIG. 4. — EB assay reveals a general blockage in cell specification. (A, B, and C) Day 9 EBs from mESCs, mESC^KDM5b, and mESC^5b+/−; arrow shows cells poorly attached to EBs. (D) qRT-PCR of KDM5b transcript from day 9 EBs. (E) qRT-PCR for Oct4 and Nanog for day 9 EBs from mESC, mESC^KDM5b, and mESCs^5b+/⁻ (primers are listed in Table S1 in the supplemental material). (F) qRT-PCR for Egr1 for day 9 EBs from mESC, mESC^KDM5b, and mESCs^5b+/⁻. (G) qsvChIP analysis for 3meH3K4 binding or KDM5b binding at the Egr1 promoter for day 9 EBs from mESC, mESC^KDM5b, and mESCs^5b+/⁻. (H) qRT-PCR of early differentiation markers from day 9 EBs. *, P = 0.05; **, P < 0.01. All data are in triplicate except where noted.