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KDM5b increases stem cell markers and proliferationa
| Marker(s) | mESC differentiation pattern
|
P value | ||
|---|---|---|---|---|
| % KDM5b | % Control | Fold change (KDM5b/control) | ||
| Oct4− Mito− | 89.74 | 95.28 | 0.9 | 7.5E-05 |
| Mito+ Oct4− | 9.26 | 4.25 | 2.2 | 0.0002 |
| Oct4+ | 0.08 | 0.08 | 1.1 | 0.9 |
| Oct4+ Mito+ | 0.93 | 0.40 | 2.3 | 0.005 |
Results of flow cytometry analysis of day 3 neurospheres showing the percentage of all live cells that stained with anti-Oct4 (Oct4−) primary/Alexa 488 secondary and/or (Mito+) anti-P-H3/Alexa 647 secondary.
