Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Jun 23;28(17):5381–5390. doi: 10.1128/MCB.00651-08

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008, American Society for Microbiology

PMC Copyright notice

FIG. 5. — Immunoblot analysis of SUMO-1, SUMO-2 and RanGAP1 proteins in wild-type and Sumo-1^−/− MEFs. (A and B) Immunoblots of lysates from wild-type and Sumo-1^−/− MEFs were generated by using anti-SUMO-1 antibody (A) or anti-SUMO-2 antibody (B). (C and D) MEFs lysates were immunoprecipitated with anti-SUMO-1 antibody (C) or anti-SUMO-2 antibody (D), after which the antibody-antigen complexes were resolved by electrophoresis on a 12% SDS gel and immunoblotted with anti-RanGAP1 antibody. IB, immunoblotting; IP, immunoprecipitation. The molecular mass of RanGAP1 is ∼70 kDa (arrow) and that of sumoylated RanGAP1 is ∼90 kDa (arrowhead).