FIG. 7.

Adipocyte differentiation of wild-type and Sumo-1^−/− MEFs. Two days after reaching the confluence, the MEFs were induced to differentiate into adipocytes. (A) Cells were stained with Oil Red at 10 days after induction. Adipocyte numbers were counted from eight randomly selected fields from four dishes and were 515 ± 125 (SEM) and 467 ± 23 (SEM) for wild-type and Sumo-null MEFs, respectively. (B) Levels of PPARγ and aP2 mRNAs in adipocytes differentiated for 10 days from wild-type and Sumo-1^−/− MEFs. “Control” refers to MEFs that were cultured in the absence of differentiation medium. The values were normalized by Gapdh mRNA levels, and the results are expressed as mean ± SEM values of three samples.