Skip to main content
. 2008 May 20;295(2):E313–E322. doi: 10.1152/ajpendo.90296.2008

Fig. 3.

Fig. 3.

PDGF expression in macrophages and adipocytes. A: PDGF mRNA in primary cells. The basal level of PDGF mRNA was determined in primary macrophages and adipocytes of lean mice. B: basal level of PDGF mRNA was determined in macrophage cell lines (RAW cells) and 3T3-L1 adipocytes. C: basal level of PDGF mRNA was determined in 3T3-L1 fibroblasts before and after differentiation into adipocytes. D: induction of PDGF mRNA expression by ambient hypoxia in primary macrophages and RAW macrophages. E: expression of PDGF in adipose tissue of lean mice after macrophage deletion. A single injection of clodronate liposome (150 mg/kg) was used to delete macrophages in lean C57BL/6J mice (8 wk old). Experiments were repeated 3 times with consistent results. Each data point represents mean ± SE (n = 3). *P < 0.05, treated vs. untreated in RAW cells; +P < 0.05, treated vs. untreated in primary macrophages.