Fig. 1.

Free radical production in murine liver after 1 h of acute acetone treatment. C57BL/B6 mice were administered a single intragastric injection of acetone (2,500 mg/kg) to mimic ketosis, and spin trapping was performed by an ip injection of α-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN, 20 mg/mouse). Representative electron paramagnetic resonance (EPR) spectra of POBN radical adducts detected in the lipid extracts of livers from acetone-treated (A) or control (B) mice 1 h after POBN injection. Spectra are representatives of at least 5 independent experiments.