Fig. 2.
Lipid radical production in the liver of mice containing the disrupted gp91phox gene (gp91phox−/−) and inducible nitric oxide synthase (iNOS) gene (iNOS−/−) or mice pretreated with the inhibitor 1400W. Lipid radical adduct production was measured by EPR spectroscopy. Knockout mice were injected intragastrically with acetone (2,500 mg/kg) and with the spin trap POBN (20 mg/mouse ip) for 1 h. Lipid radical adducts were determined in the lipid extracts of liver samples. Each experiment was made in quadruplicate. Representative EPR spectra of lipid radicals detected in the liver of acetone-treated C57BL mice (A), acetone-treated NADPH oxidase knockout mice (B), acetone-treated iNOS knockout mice (C), and acetone-treated C57Bl mice pretreated with the iNOS inhibitor 1400W (15 mg/kg) (D).