FIG. 1.

The NADPH oxidase genes nox-1 and nox-2 are differentially expressed during growth and sexual development. RNA was extracted from conidia (C) or mycelia from wild-type strain 74-ORS23-1A grown in liquid culture (A) or mycelia induced to undergo sexual development for the indicated time (hours) (B) and was hybridized with nox-1- or nox-2-specific probes. Bottom panels show rRNA as loading reference. To induce sexual development, conidia were grown on plates with solid crossing medium covered with a cellophane membrane, and samples were harvested at the indicated times.