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Use of real time PCR to estimate the relative abundances of ALG5 and DPM1 mRNAs in cultured Trichomonas (see Table S1 in the supplemental material for the list of primers). A log scale shows that the TvALG5D and TvALG5E mRNAs are much more abundant than those of TvALG5A to -C and TvALG5G, which in turn are much more abundant than that of TvDPM1. Calibrations were relative to a Trichomonas actin gene.
