FIG. 2.

Interactions between TORC1 components are not affected in avo3^ts mutants. Strains expressing a Myc-tagged TORC1 component were subjected to the same procedures as those for Fig. 1. Immunoprecipitates were examined by Western blot analysis using anti-Tor1p antibodies. Expression of Tor1p in the lysates is shown at the bottom of each panel. WT, wild type; BC, bead control; IP, immunoprecipitation; CoIP, coimmunoprecipitation. (A) Tor1p-Lst8p interaction detected in YMY118 (WT), YMY216 (avo3-1^ts), and YMY316 (avo3-2^ts). (B) Tor1p-Kog1p interaction detected in YMY119 (WT), YMY217 (avo3-1^ts), and YMY317 (avo3-2^ts).