FIG. 2.

Defect in adaptation to arsenite-induced G₁ checkpoint arrest is not solely caused by increased accumulation of arsenite. The acr3Δ mutant lacking the arsenite efflux transporter and exhibiting elevated levels of arsenite in the cytosol shows prolonged but not persistent G₁ arrest, as shown by the results of flow cytometry in asynchronous (A) and G₁-synchronized cells (B), as well as by the results of the α-factor-nocodazole trap assay (C). As(III), arsenite; +, present; −, absent; 1C and 2C, values of DNA content; WT, wild type.