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A: Signaling by oncogenic kRas interferes with apoptosis induced by DHCB. A:Apoptosis was determined in HCT116 and Hke-3 cells that were treated with increasing concentrations of DHCB for 24, 48 or 72 hours by PI staining. Error bars were derived from 3 experiments. B: The activation of caspase 9 was determined in cell lysates using antibody that recognizes both full length caspase 9 and cleaved caspase 9, as well as with antibody recognizing specifically cleaved caspase-9.
