Table 1.
Effect of sample injection time (Tinj) on migration time (Tmig), width at half height (W1/2), efficiency (N), peak height, and peak area for dofetilide in Ringer’s solution without acid stacking, with acid stacking, and with acid stacking and reverse pressure
| Tinj (s) sample/acid | Tmig (s) | W1/2 (s) | N/1000 | Peak height (mAU) | Peak area/1000 |
|---|---|---|---|---|---|
| Ringer’s solution without acid stacking | |||||
| 5 | 320.2 ± 4.8 | 2.8 ± 0.1 | 72.1 ± 3.3 | 2.3 ± 0.2 | 6.8 ± 0.6 |
| 10 | 322.5 ± 6.2 | 2.6 ± 0.2 | 85.5 ± 7.4 | 3.8 ± 0.3 | 14.1 ± 1.1 |
| 20 | 321.9 ± 3.4 | 3.1 ± 0.4 | 59.2 ± 9.2 | 5.9 ± 0.4 | 23.8 ± 1.9 |
| 30 | 322.0 ± 4.9 | 4.3 ± 0.6 | 31.7 ± 3.8 | 6.5 ± 0.5 | 34.4 ± 2.9 |
| 40 | 324.5 ± 7.1 | 6.8 ± 0.6 | 13.4 ± 1.4 | 6.7 ± 0.6 | 45.3 ± 4.4 |
| With acid stacking | |||||
| 5/8 | 286.1 ± 2.4 | 1.4 ± 0.1 | 231.2 ± 5.9 | 3.2 ± 0.1 | 6.6 ± 0.4 |
| 10/16 | 288.3 ± 1.8 | 1.6 ± 0.1 | 179.6 ± 8.2 | 5.9 ± 0.2 | 12.2 ± 0.8 |
| 20/32 | 291.7 ± 2.2 | 1.7 ± 0.2 | 162.4 ± 7.7 | 14.4 ± 1.0 | 25.1 ± 1.1 |
| 30/48 | 285.0 ± 2.1 | 1.6 ± 0.1 | 176.7 ± 4.9 | 19.6 ± 1.4 | 34.9 ± 1.4 |
| 40/64 | 297.7 ± 2.9 | 2.0 ± 0.1 | 121.9 ± 2.5 | 21.9 ± 3.2 | 46.8 ± 1.6 |
| 50/80 | 299.3 ± 4.0 | 2.5 ± 0.2 | 79.0 ± 4.7 | 24.3 ± 6.1 | 61.2 ± 2.1 |
| 60/96 | 313.6 ± 3.6 | 3.0 ± 0.1 | 60.5 ± 2.8 | 22.7 ± 3.5 | 72.0 ± 3.2 |
| 70/112 | 315.2 ± 5.1 | 3.6 ± 0.2 | 42.7 ± 2.5 | 22.9 ± 4.7 | 83.7 ± 3.9 |
| 90/144 | 312.2 ± 5.4 | 4.8 ± 0.3 | 23.1 ± 2.2 | 21.6 ± 4.6 | 108.5 ± 7.3 |
| 120/192 | 314.8 ± 7.8 | 5.9 ± 0.5 | 16.3 ± 1.8 | 23.9 ± 5.4 | 141.8 ± 11.8 |
| With acid stacking and reverse pressure | |||||
| 5/8 | 316 ± 2.1 | 1.5 ± 0.1 | 246.2 ± 9.1 | 3.4 ± 0.2 | 6.2 ± 0.4 |
| 10/16 | 315 ± 2.3 | 1.4 ± 0.2 | 280.8 ± 9.4 | 6.7 ± 0.3 | 11.9 ± 0.9 |
| 20/32 | 321 ± 1.9 | 1.7 ± 0.1 | 198.3 ± 7.7 | 12.1 ± 0.4 | 23.8 ± 1.4 |
| 30/48 | 321 ± 2.4 | 1.9 ± 0.1 | 158.1 ± 5.8 | 17.3 ± 1.7 | 35.9 ± 1.8 |
| 40/64 | 322 ± 2.2 | 2.2 ± 0.2 | 119.0 ± 3.6 | 18.7 ± 3.0 | 48.4 ± 2.0 |
| 50/80 | 324 ± 3.4 | 2.4 ± 0.1 | 101.6 ± 4.9 | 22.4 ± 4.6 | 61.1 ± 2.7 |
| 60/96 | 326 ± 3.2 | 2.5 ± 0.1 | 94.0 ± 2.8 | 25.5 ± 5.2 | 71.7 ± 3.5 |
| 70/112 | 325 ± 5.1 | 2.3 ± 0.1 | 111.7 ± 3.3 | 31.9 ± 5.4 | 84.3 ± 4.3 |
| 90/144 | 327 ± 8.6 | 2.8 ± 0.2 | 76.4 ± 2.3 | 33.6 ± 6.1 | 108.5 ± 7.0 |
| 120/192 | 329 ± 9.6 | 3.2 ± 0.1 | 59.2 ± 3.1 | 38.2 ± 5.7 | 144.0 ± 7.5 |
| 150/240 | 330 ± 8.2 | 3.6 ± 0.1 | 47.3 ± 3.4 | 43.7 ± 6.3 | 177.9 ± 12.2 |
| 180/288 | 334 ± 9.3 | 4.8 ± 0.3 | 27.9 ± 3.6 | 37.6 ± 4.9 | 216.4 ± 15.3 |
| 210/336 | 338 ± 9.0 | 5.6 ± 0.3 | 20.2 ± 3.2 | 39.2 ± 5.8 | 253.8 ± 19.6 |
| 240/384 | 342 ± 9.1 | 6.1 ± 0.5 | 17.4 ± 2.4 | 41.5 ± 5.2 | 285.8 ± 23.1 |
Values are presented as mean ± standard deviation from experiments run in triplicate.