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. 2008 Sep 5;3(9):e3152. doi: 10.1371/journal.pone.0003152

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Buijs et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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TNP-OVA IgM antibody production after i.v. injection of saline and TNP-OVA (dashed lines) or LPS and TNP-OVA(uninterrupted lines) in animals that sustained a parasympathetic denervation (red) or a sympathetic denervation (yellow) of the spleen or were sham operated (blue). Only parasympathetic denervation results in a nearly complete loss of antibody production with a similar response as if no LPS were given. (Two way ANOVA indicated difference between the sham and sympathetic denervation + LPS with the other groups without LPS and the parasympathetic denervated group with LPS p<0.01. Tukey post hoc test showed significant difference of day 7 and 10 of the sham and sympathetic denervation as compared to all other time points p<0.001.)