Fig. 3.

Increasing hepatic HNF-6 expression enhances in vivo hepatocyte proliferation. Livers were immunostained for 5-bromo-2-deoxyuridine (BrdU) incorporation. Hepatocyte proliferation were graphed and reported as means ± SD percent of BrdU-positive hepatocytes in 1) Sham/PBS or Sham/GH for 1 or 7 days (n = 4; A) showing that GH enhanced spontaneous hepatocyte proliferation; 2) mock-, AdLacZ-, or AdHNF-6-treated liver (n = 4; B), showing that HNF-6 overexpression enhanced hepatic regeneration; and 3) PBS- or GH- treated Sham or BDL day 1 or 7 liver (C), showing increases in hepatocyte proliferation in GH-treated liver. Sham data (A) are replotted for comparison.