Fig. 4.

GH induces cyclin D1 expression in uninjured and BDL liver. A and B: real-time PCR analyses of PBS- or GH- treated day 1 (A) or 7 (B) Sham liver showed that increased HNF-6 expression in GH-treated Sham liver was associated with upregulation of Foxm1b and cyclin D1 but not TGF-α gene expression. C and D: PBS- or GH- treated day 1 (C) or 7 (D) liver gene expression showed that increased HNF-6 expression in GH/BDL liver was associated with upregulation of cyclin D1 but not Foxm1b or TGF-α expression. E: representative Western blot micrographs of day 1 and 7 Sham/GH, PBS/BDL, and GH/BDL liver showed enhanced cyclin D1 protein expression in GH/BDL liver relative to PBS/BDL liver at both time points.