Table 1.

Oligonucleotides designed from genes of interest and utilized for real-time PCR

Protein	Gene ID	Reference Sequence	mRNA Coordinates	Primer	5′-oligonucleotide sequence-3′	Amplicon Size, bp
NHE1	Slc9a1	NM_012652	1992–2011	Forward	CACCAGTGGAACTGGACCTT	211
			2202–2183	Reverse	ACATGGGGAAGTGCTTCTTG
NHE2	Slc9a2	NM_012653	2173–2192	Forward	CGCAGGGCTTCTACTTCAAC	235
			2407–2388	Reverse	CGTCCACCTCATTCTTCCAT
NHE3	Slc9a3	NM_012654	1885–1905	Forward	GGAACAGAGGCGGAGGAGCAT	322
			2206–2187	Reverse	GAAGTTGTGTGCCAGATTCT
ENaC-α	Scnn1a	NM_031548	1443–1462	Forward	TACCCTAAGCCCAAGGGAGT	226
			1668–1649	Reverse	TGTTCTGCAAGGACAGCATC
ENaC-β	Scnn1b	NM_012648	1505–1524	Forward	ACCCTGAGCAGGAAGGGTAT	222
			1726–1707	Reverse	ACAGGAGGCCACTAGCTTGA
ENaC-γ	Scnn1 g	NM_017046	680–699	Forward	GCACGTTCATGAGTCGAAGA	206
			885–866	Reverse	AGAAGCAGGTCACCAGCAGT
Na+/K+-ATPase	Atp1a1	NM_012504	3207–3231	Forward	TGCCTTCCCCTACTCCCTTCTCATC	323
(α 1 subunit)			3529–3505	Reverse	CTTCCCCGCTGTCGTCCCCGTCCAC
AT1	Agtr1a	NM_030985	392–411	Forward	GGAAACAGCTTGGTGGTGAT	241
			632–614	Reverse	CGATGCTGAGACACGTGAG
AT2	Agtr2	NM_012494	252–272	Forward	CAATCTGGCTGTGGCTGACTT	101
			352–331	Reverse	TGCACATCACAGGTCCAAAGAG
GAPDH	Gapd	NM_017008	1270–1289	Forward	TCCCTCAAGATTGTCAGCAA	308
			1577–1558	Reverse	AGATCCACAACGGATACATT

NHE, sodium-hydrogen exchanger; ENaC, epithelial sodium channel.