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Cre-mediated recombination is dose dependent. ROSA26R reporter mice were injected with increasing dilutions of MUP-iCre-AAV8. Livers were harvested after 1 wk, and β-galactosidase staining was performed on sections. A: 1.4 × 1010 DNase-resistant particles (DRP)/mouse. B: 1.4 × 109 DRP/mouse. C: 0.5 × 109 DRP/mouse. D: empty MUP-AAV8 (2.2 × 1011 DRP/mouse). Original magnification 400×.
