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. Author manuscript; available in PMC: 2008 Aug 25.
Published in final edited form as: Biochemistry. 2006 Apr 11;45(14):4371–4377. doi: 10.1021/bi0525268

Figure 4. Acid-induced unfolding of 5-helix fragments of bacterio-opsin.

Figure 4

Fraction of folded protein measured by FRET from tryptophan donors to dansyl acceptors. Acceptors: heavy solid line (circles), dansyl groups on Cys 163 (EF loop) of fragment C1 (residues 72-248); dashed line (triangles), dansyl groups on Lys 41 (helix B) of fragment V1 (residues 1-166); thin line, curve fit for Lys 41 label on intact bacterio-opsin (same as solid line in fig. 3). Conditions same as fig. 3.