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. Author manuscript; available in PMC: 2008 Aug 25.
Published in final edited form as: Biochemistry. 2006 Apr 11;45(14):4371–4377. doi: 10.1021/bi0525268

Figure 5. Apparent association of V1 and V2 fragments, measured by FRET.

Figure 5

V1-V2 association data from magnitude of sensitized emission at 285 nm, analyzed as described in text. Line calculated from eq. (1) with association constant = 0.017 μM−1. Error bars indicate standard deviation from line. Conditions, 0.7 μM V1, 0.55% CHAPS, 0.55% DMPC, 0.15% SDS, 0.05 M phosphate, pH 6.0.