. Author manuscript; available in PMC: 2008 Aug 25.

Published in final edited form as: Biochemistry. 2006 Apr 11;45(14):4371–4377. doi: 10.1021/bi0525268

Table 1.

pH* dependence of acid-induced unfolding of BO

Protein	FRET acceptor site	modification reaction	apparent pK	ΔΔG_u (kcal/mol)
BO	K 41	dansyl chloride	4.75	^a
BO	C 163	didansyl cystine	5.15	−0.54
BO	C 222	didansyl cystine	5.75	−1.4
C1 fragment	C 163	didansyl cystine	5.46	−1.0
V1 fragment	K 41	dansyl chloride	7.36	−3.5

other entries in this column calculated relative to data for K41 site in BO, using equation 6.