Table 2.
Characterization of mutant polioviruses
| Parental Virus | Time for complete CPE | Mutations in recovered viruses | |
|---|---|---|---|
| A | wt | 24 h | N.D. |
| B | W42F | 24 h | W42F |
| C | F69W | 40 h | F69W |
| D | W42F/F69W | 7 days | W42F/F69W/A70V + 2B I47V |
| E | W42F/F69W/A70V | 7 days | W42F/F69W/A70V + 2B I47V |
| F | 2B I47V | 26 h | N.D. |
| G | W42F/F69W/A70V + 2B I47V | 48 h | N.D. |
| H | W42F/V72W | 48 h | T14K/W42F/V72W*
P17A/W42F/V72W* |
| I | W42F/A73W | 72 h | W42F/A73C |
| W42F/G74W | 48 h | W42F/G74C*
W42F/G74S* |
|
| K | W42F/V75W | 60 h | W42F/V75C |
All mutations shown map to polypeptide 3A with the exception of I47V, which maps to polypeptide 2B (see Fig. 1). CPE: cytopathogenic effect; N.D.: not determined;
*
Two genetic variants that emerged independently.