Table 3.
Quenching of Membrane-Inserted Anchor Peptide-1 and Anchor Peptide-2 by Acrylamide and 10-Doxylnonadecane.
| Peptide | Fo/F Acrylamide | Fo/F 10-DN | Q-Ratio | λmax (nm) |
|---|---|---|---|---|
| Peptide inserted into DOPC vesicles (C18:1 acyl chains) a | ||||
| Anchor Peptide -1 | 1.31 ± 0.02 | 1.88 ± 0.01 | 0.352 ± 0.02 | 332± 0.2 |
| Anchor Peptide-2 | 1.25 ± 0.01 | 2.33 ± 0.14 | 0.192 ± 0.21 | 327± 0.25 |
| Peptide inserted into DEuPC vesicles (C22:1 acyl chains) | ||||
| Anchor Peptide-1 | 2.11 ± 0.11 | 2.29 ± 0.13 | 0.82 ± 0.15 | 339.8± 0.4 |
| Anchor Peptide-2 | 1.91 ± 0.02 | 1.99 ± 0.04 | 0.92 ± 0.05 | 340± 0.3 |
a
Fo/F is the ratio of fluorescence in the absence of quencher to the that in the presence of quencher. The ratio of quenching by acrylamide to that by 10-DN, the Q-ratio, is defined in Experimental Procedures. Average values and standard deviations derived from six samples are shown.