Table 1.
Percent Mössbauer absorptions of the Fe species detected in the as-prepared, cryoreduced and high-temperature annealed oxy-HO and oxy-Mb samples
| Oxy-HO
|
Oxy-Mb
|
||||
|---|---|---|---|---|---|
| Condition | Species | % absorptiona | Condition | Species | % absorptiona |
| As-prepared | FeII-O2 | 84(5) | As-prepared | FeII-O2 | 76(3) |
| FeIII-H2O | 16(5) | FeIII-H2O | 24(3) | ||
|
| |||||
| Reduced at 77K | FeII-O2 | 36(3) | Reduced at 77K | FeII-O2 | 27(3) |
| FeIII-H2O | 10(2) | FeIII-H2O | 10(2) | ||
| FeIII-O2H− | 34(6) | FeIII-O22− | 48(5) | ||
| S = 0 FeII | 14(3) | S = 0 FeII | 15(3) | ||
| S = 1 FeIV | 4(2) | ||||
|
| |||||
| Annealed at 180 K | Same as prior to annealing | Annealed at 180 K | FeII-O2 | 25(3) | |
| FeIII-H2O | 10(2) | ||||
| FeIII-O22− | 50(7) | ||||
| S = 0 FeII | 14(2) | ||||
|
| |||||
| Annealed at 220 K | FeII-O2 | 33(4) | Annealed at 195 K | FeII-O2 | 25(3) |
| FeIII-H2O | 9(5) | FeIII-H2O | 11(5) | ||
| FeIII-O2H− | 9(4) | FeIII-O22− | 5(5) | ||
| α-hydroxyhemin | 22(5) | FeIV=O2− | 27(3) | ||
| S = 0 FeII | 6(2) | FeIII-product | 18(5) | ||
| S = 2 FeII | 4(3) | Deoxy Mb | 14(2) | ||
| S = 1 FeIV | 6(2) | ||||
a
values in the parentheses are uncertainties estimated for the last significant digits.