Figure 4.

Fractionation of pooled 26S proteasome-containing fractions from a Sephacryl S-400 column by Mono Q anion exchange chromatography. (A) Peptidase activities in fractions collected from a Mono Q HR (5/5) anion exchange column. Elution of proteins was with a linear gradient of 26S buffer containing 0.8 M NaCl (buffer B). (B) Immunoblot analysis of HA-Doa4 and Cim5 proteins in the Mono Q fractions. Asterisk, weak anti-HA cross-reactivity with an abundant yeast protein (not always observed).