Figure 8.

Expression of chimeric Doa4-Ubp5 proteins in doa4Δ cells. (A) Schematics of chimeric DOA4-UBP5 genes specifying the sites of fusion. Lightly stippled boxes correspond to DOA4 coding sequences, and darkly stippled boxes correspond to those of UBP5. Doa4 function was assayed by growth of doa4Δ haploid cells on 0.8 μg/ml canavanine and by sporulation of doa4Δ/doa4Δ diploid cells. (B) Anti-HA immunoblot analysis of extracts from doa4Δ cells expressing the chimeras diagrammed in A and from cells expressing Ubp5-HA (lane marked Ubp5).