Figure 9.

Close correlation between Deg1-βgal accumulation and levels of low molecular mass (poly)ubiquitin conjugates in doa4Δ cells expressing Doa4-Ubp5 chimeras. (A) βgal activity assays of extracts made from yeast cells with an integrated Deg1-lacZ reporter gene. W.T., wild-type strain (MHY501). The remaining strains harbor a chromosomal deletion of DOA4 and carry the empty YEplac195 vector (doa4) or plasmids expressing Ubp5 or the Doa4-Ubp5 chimeras. Numbering of chimeras as in Figure 8. (B) Antiubiquitin immunoblot analysis of same strains as in A.