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Purification of yeast 26S proteasomes
| Step | Protein (mg/ml) | Volume (ml) | Total protein (mg) | Total activity (U)a | Specific activity (U/mg) | Fold-purified |
|---|---|---|---|---|---|---|
| Crude | 12.0 | 6.0 | 72 | 4379 | 61 | |
| S-400 pool 36–44 | 0.13 | 50 | 6.25 | 15680 | 2509 | 41 |
| Mono-Q pool 33–34 | 1.23 | 0.8 | 0.98 | 6680 | 6816 | 112 |
| Superose 6 pool 32–36 | 0.08 | 1.5 | 0.12 | 3732 | 31100 | 512 |
One unit is defined as the fluorescence signal of a reaction catalyzed by 1 μl of enzyme in 1 min when suc-LLVY-AMC is in excess (integration time = 1 s).
