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. 1999 Mar;10(3):741–756. doi: 10.1091/mbc.10.3.741

Table 2.

ATP-dependence of ubiquitin–[125I]-lysozyme conjugate degradation by purified yeast 26S proteasomes

Reaction Enzyme ATPa % degradationb
1 Superose 6 fraction 33 + 19.1
2 Superose 6 fraction 33 − 10.8
3 Buffer + 10.6
a

ATP regeneration system (+): 5 mM ATP, 50 mM creatine phosphate, 50 μg/ml creatine kinase; ATP depletion system (−): 25 mM glucose, 25 μg/ml hexokinase. 

b

Average of two independent reactions (45 min).