Fig. 2.

A: low magnification photomicrograph of a hypothalamic section (approximating Plate 38–39, Ref. 40) stained for PRV and for melanocortin 4-receptor (MC4-R) mRNA and then counterstained with Cresyl Violet. B: portion of the paraventricular nucleus of the hypothalamus (PVH) outlined in Fig. 2A is enlarged. Curved open arrow = PRV (brown); straight black arrow = MC4-R mRNA (autoradiography black granules); curved black arrows = [MC4-R + PRV] cells (black granules over brown DAB). Arrows indicate representative cells within the section. ZI, zona incerta; SCH, suprachiasmatic nucleus; 3V, third ventricle; OC, optic chiasm. Bar = 25 μM.