Fig. 4.

A: photomicrograph of hamster brain stem section processed for PRV and MC4-R mRNA (approximating Plate 85, Ref. 40). Areas outlined in Fig. 4A are enlarged for cellular resolution of the single- and double-labeled cells in the raphé pallidus (RPA; B), rostroventrolateral reticular nucleus (RVL; C), and raphé obscurus (ROb; B). Single-labeled PRV (curved open arrow), single-labeled MC4-R mRNA (straight black arrow), and double-labeled [MC4-R + PRV] (curved black arrows) cells can also be seen. Arrows indicate representative cells. GiA, gigantocellular reticular nucleus, alpha; LPGi, lateral paragigantocellular nucleus. Bar = 25 μM.