Table 1.

Results of a pilot study to determine third ventricular injections of melanotan II (MTII) that do not increase interscapular brown adipose tissue (IBAT) temperature

Dose	1 h Postinjection	2 h Postinjection	3 h Postinjection	4 h Postinjection	No.
Saline	36.8±0.2	37.3±0.2	37.3±0.2	37.1±0.2	12
MTII, 0.05 nmol	37.0±0.6	37.1±0.8	37.0±0.3	36.9±0.4	4
MTII, 0.10 nmol	37.2±0.2	37.3±0.1	37.2±0.2	36.8±0.2	6
MTII, 0.20 nmol	37.2±0.2	37.3±0.5	37.5±0.4	37.2±0.1	3
MTII, 0.30 nmol	37.8±0.3	37.7±0.3	37.0±0.5	36.8±0.3	3

Note that we previously reported that third ventricular injections of 5 nmol MTII maximally stimulated IBAT temperature, with a smaller, but significant increase in IBAT temperature at 0.5 nmol (11); therefore, we began testing with doses lower than this latter dose.