Table 2.
Distribution of PRV and PRV + MC4-R mRNA after IBAT injection of PRV
| PRV | [PRV + MC4-R]÷PRV% | PRV | [PRV + MC4-R]÷PRV% | ||||
|---|---|---|---|---|---|---|---|
| Forebrain | VLPAG | 599.4±200.67 | 82.64±0.6 | ||||
| Pontine | 464.20±209.81 | 78.48±2.15 | |||||
| Hypothalamic | PPTg | 322.20±146.74 | 85.79±3.82 | ||||
| AH/LA | 102.80±38.14 | 76.08±3.29 | pv | 20.75±10.86 | 38.03±19.94 | ||
| Arc | 108.20±44.68 | 77.86±6.15 | R | 64.00±32.87 | 83.47±2.66 | ||
| DM | 181.20±73.88 | 79.78±5.07 | RRF/A8/rs | 26.75±14.14 | 59.97±19.13 | ||
| DTM | 17.75±4.84 | 90.03±3.25 | RtTg | 12.50±2.21 | 90.63±5.93 | ||
| LH | SN | 12.20±5.62 | 87.39±6.52 | ||||
| SUBZi | 490.00±161.31 | 80.46±1.28 | SPTg | 55.00±17.06 | 81.16±3.28 | ||
| Pe/AVPe | 417.40±113.54 | 82.84±1.97 | VLTg | 27.33±4.84 | 81.33±8.46 | ||
| PH | 219.60±87.70 | 85.74±4.53 | VTA | 201.00±55.48 | 88.76±2.10 | ||
| POA | 589.40±230.30 | 76.79±1.54 | Xscp | 12.75±9.68 | 84.52±6.05 | ||
| PVN | 1172.60±308.83 | 84.22±2.02 | Brainstem | ||||
| PaAP | 48.20±20.80 | 79.70±3.78 | |||||
| PaDC | 20.50±6.64 | 85.16±3.26 | 12 | 9.67±2.70 | 79.63±3.59 | ||
| PaLM | 183.60±50.95 | 83.41±3.37 | 10 (DMV) | 22.50±0.95 | 86.90±2.26 | ||
| PAMM | 374.20±122.87 | 83.23±2.91 | A5 | 285.40±91.51 | 79.47±1.52 | ||
| PaMP | 204.20±86.87 | 82.65±2.65 | Amb | 135.75±52.00 | 81.07±2.12 | ||
| PaPo | 366.25±148.94 | 85.59±3.95 | AP | 71.40±21.19 | 72.14±2.21 | ||
| PaV | 61.20±18.09 | 80.27±5.05 | Bar | 50.75±25.97 | 81.60±2.39 | ||
| RCh | 14.33±7.28 | 73.30±3.47 | Facial | 280.60±91.42 | 76.72±2.49 | ||
| Spa | 10.67±3.80 | 74.32±10.42 | DPGi | 117.00±21.19 | 80.12±2.87 | ||
| SCH | 30.67±6.93 | 68.51±1.49 | LPGi | 678.20±166.75 | 84.34±1.92 | ||
| VMH | 74.00±23.38 | 71.52±1.76 | Gi | 482.00±153.16 | 86.90±4.35 | ||
| Thalamic | GiA | 274.80±72.38 | 83.05±2.27 | ||||
| GiV | 316.20±68.24 | 91.98±1.16 | |||||
| LHb | 14.50±3.48 | 81.94±4.39 | KF | 53.75±8.66 | 82.66±5.24 | ||
| PrC | 58.00±25.97 | 77.97±1.11 | LC | 168.40±60.17 | 80.67±2.31 | ||
| PSTh | 23.50±5.38 | 80.63±0.40 | Li | 84.33±8.05 | 90.54±0.49 | ||
| PV | 18.25±5.14 | 93.84±3.95 | LPB | 100.75±31.63 | 75.30±7.74 | ||
| Re/Vre/Xi | 139.75±55.63 | 81.71±2.53 | MPB | 82.20±24.60 | 82.99±5.24 | ||
| Rt | 12.00±3.66 | 86.83±2.65 | PBP | 49.00±15.81 | 82.71±7.51 | ||
| Other Forebrain | Ppy | 53.25±16.81 | 76.32±2.79 | ||||
| Pr | 196.40±45.77 | 78.78±5.72 | |||||
| AC | 11.00±2.93 | 74.07±6.75 | |||||
| Amygdala | 14.50±1.58 | 88.24±7.44 | Raphé | ||||
| BST | 114.00±56.58 | 83.70±2.16 | DR | 248.60±94.86 | 79.45±5.50 | ||
| HDB/VDB | 18.50±6.64 | 70.91±5.32 | MnR | 13.50±2.85 | 77.78±0.00 | ||
| Fascicular | 26.40±9.91 | 50.49±13.42 | PMR | 12.40±10.43 | 97.78±2.22 | ||
| LS | 5.00±1.32 | 93.30±3.47 | Rli/Cli | 23.60±9.36 | 78.03±5.25 | ||
| PeF | 17.50±2.21 | 85.71±9.04 | RC | 208.80±65.15 | 82.97±2.79 | ||
| Mammillary bodies | 20.80±16.67 | 85.47±9.19 | RMg | 224.40±67.06 | 76.33±2.93 | ||
| SI | 14.50±4.74 | 86.36±8.62 | Rob | 343.00±85.00 | 68.57±17.16 | ||
| TC | 38.00±8.93 | 69.73±6.89 | Rpa | 605.80±97.51 | 85.16±2.32 | ||
| ZI | 60.50±24.87 | 87.03±5.83 | Reticular | ||||
| Midbrain | Md-D/V | 165.20±70.57 | 85.06±3.85 | ||||
| CnF | 98.20±70.48 | 81.65±6.20 | Irt | 292.60±120.97 | 81.22±1.96 | ||
| Dk | 19.50±4.74 | 96.30±2.34 | LRt | 376.80±99.86 | 82.25±1.85 | ||
| DMTg | 53.25±20.92 | 78.02±2.97 | PCRt | 42.33±18.62 | 86.39±5.27 | ||
| DpMe | 267.60±88.84 | 87.53±3.42 | PCRtA | 67.40±18.70 | 82.11±5.13 | ||
| DTg | 18.50±11.07 | 97.22±1.76 | PMn | 111.80±60.93 | 86.43±4.19 | ||
| EW | 5.00±0.37 | 76.67±7.89 | RVL | 229.40±80.62 | 76.97±3.79 | ||
| F | 1.00±0.00 | 50.00±31.62 | Sol | 532.80±152.44 | 82.40±4.60 | ||
| LDTg/-V | 188.40±41.63 | 85.14±2.78 | Sub-cd/v | 123.80±49.50 | 81.40±5.47 | ||
| Lemniscus | 77.00±46.18 | 79.46±5.36 | Trigeminal | 295.40±70.91 | 80.80±1.52 | ||
| Oculomotor | 52.80±25.47 | 89.82±3.83 | Vestibular | 90.40±38.88 | 77.51±2.98 | ||
| Olivary | 95.60±49.91 | 82.85±5.79 | Vsc | 1027.20±293.29 | 73.89±3.62 | ||
| Pa4 | 15.00±8.22 | 94.64±3.39 | |||||
| Pc/Pcom | 30.80±14.80 | 87.18±4.99 | |||||
| PAG | 1337.60±491.95 | 82.51±0.87 | |||||
| DLPAG | 85.5±38.42 | 78.39±4.26 | |||||
| DMPAG | 229.6±87.90 | 85.16±4.07 | |||||
| LPAG | 308.2±131.73 | 85.90±3.62 | |||||
Values are means ± SE. PRV, pseudorabies virus; MC4-R, melanocortin 4 receptors; AH, anterior hypothalamus; Arc, arcuate nucleus; DM, dorsomedial nucleus; DTM, dorsal tuberomammillary; LH, lateral hypothalamus; Pe/AVPe, periventricular/anteroperiventricular; PH, posterior hypothalamus; POA, preoptic area; PVN, paraventricular hypothalamus; PaAP, paraventricular hypothalamic anterior parvicellular; PaDC, parventricular hypothalamic dorsal cap; PaLM, parventricular hypothalamic lateral magnocellular; PaMM, paraventricular hypothalamic medial magnocellular; PaMP, paraventricular hypothalamic medial parvocellular; PaPo, paraventricular hypothalamic posterior; PaV, paraventricular hypothalamic ventral part; RCh, retrochiasmatic area; Spa, subparaventricular nucleus; SCH, suprachiasmatic nucleus; SubZi, sub-zona incerta of the LH; VMH, ventromedial hypothalamus; LHb, lateral habenular; PrC, precommissural nucleus; PSTH, parasubthalamic nucleus; PV, parventricular thalamus; Re/Vre/Xi, reuniens/reuniens ventral/xiphoid; Rt, reticular thalamic nucleus; AC, anterior commissural nucleus; BST, bed nucleus of the stria terminalis; HDB/VDB, diagonal band-horizontal limb/vertical limb; LS, lateral septum; ns, nigrostriatal bundle; PeF, perifornical nucleus; SI, substantia innominata; TC, tuber cinerium; ZI, zona incerta; A8, dopaminergic cell field; CnF, cuneiform; Dk, darkschewitsch; DMTg, dorsomedial tegmental area; DpMe, deep mesencephalic nucleus; DTg, dorsal tegmental; EW, Edinger Westphal; F, nucleus of the fields of Forel; LDTg/-V, laterodorsal tegmental/-ventral part; Pa4, paratrochlear; Pc/Pcom, postcommissural/nucleus; PAG, periaqueductal gray; PPTg, pedunculopontine tegmental nuclei; pv, periventricular fiber system; R, red nucleus; RRF, retrorubral field; rs, rubrospinal tract; RtTg, reticulo temental pons; SN, substantia nigra; SPTg, subpeduncular tegmental nucleus; VLTg, ventrolateral tegmental nucleus; VTA, ventral tegmental area; xscp, decussation of the superior cerebellar peduncle; 10 DMV, dorsal motor nucleus of the vagus nerve; 12, hypoglossal nucleus; A5, noradrenergic cell field; Amb, ambiguous nucleus; AP, area postrema; Bar, Barrington's nucleus; DPGi, dorsal paragigantocellular nucleus; Gi, gigantocellular reticular nucleus; GiA, gigantocellular reticular anterior; GiV, gigantocellular reticular ventral; KF, Kolliker fuse; LC, locus coeruleus; Li, linear nucleus of the medulla; LPB, lateral parabrachial nucleus; LPGi, lateral paragigantocellular nucleus; MPB, medial parabrachial nucleus; PBP, parabrachial pigmented; Ppy, peripyramidal nucleus; Pr, prepositus hypoglossal nucleus; DR, dorsal raphé; MnR, median raphé; PMnR, paramedian raphé; RC, raphé cap; Rli/Cli, rostral linear nucleus of the raphé/caudal linear nucleus of the raphé; RMg, raphé magnus nucleus; ROb, raphé obscurus; RPa, raphé pallidus; Irt, intermediate reticular nucleus; LRt, lateral reticular nucleus; MdD-/V, medullary nucleus dorsal-/ventral; PCRt-/A, parvicellular reticular nucleus/anterior; PMn, paramedian reticular nucleus; RVL, rostroventrolateral reticular nucleus; Sol, solitary tract nucleus; SubCD/-V, subcoeruleus nucleus; dorsal/-ventral; vsc, ventral spinocerebellar tract.