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. 2008 Jun 25;295(2):R371–R380. doi: 10.1152/ajpregu.00468.2007

Fig. 3.

Fig. 3.

Renal renin expression, plasma renin activity, and systemic blood pressure in C57BL/6 and Cx45fl/fl:Nestin-Cre mice. A: C57BL/6 (control, n = 5) and Cx45fl/fl:Nestin-Cre (n = 4) kidney homogenate samples were blotted with renin antibodies. B: densitometric analysis of immunoblot indicated that the expression of renin in Cx45fl/fl:Nestin-Cre mice was significantly higher than that of C57BL/6 mice (*P < 0.05). C: plasma renin activity was measured in real-time using a fluorescence resonance energy transfer-based fluorogenic renin substrate. Renin activity was significantly higher in Cx45fl/fl:Nestin-Cre mice compared with C57BL/6 mice, and this increase was comparable to the increase observed in captopril-treated mice (*P < 0.05, n = 5 per group). D: mean arterial blood pressure was significantly increased by ∼30% in Cx45fl/fl:Nestin-Cre mice (*P < 0.05, n = 3 per group). Values are means + SE.