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A: Gαq protein levels normalized to GAPDH expressed as optical density units/mm2 in CNS sites in Sprague-Dawley rats pretreated (24 h) with intracerebroventricular Gαq ODN (25 μg, n = 6) or isotonic saline vehicle (5 μl, n = 6). *P < 0.05 compared with control. B: representative immunoblots for GAPDH, Gαq, and Gαz taken from a hypothalamic sample from a male Sprague-Dawley rat pretreated (24 h) with intracerebroventricular Gαq ODN (25 μg) or saline vehicle (5 μl).
