Table 7.
Statistical significance and strain ranking consistency for selected variables in the tests performed
| Test | Measure | Strain | Center | Strain × Center | Strain Ranking Consistency (# of Centers) |
|---|---|---|---|---|---|
| Open field | distance traveled | P<0.0001 | P<0.0001 | P=0.0012 | C57>C3H, BALB>129 (4/4) |
| Open field | % time in center | P<0.0001 | P<0.0001 | P<0.0001 | C57>C3H>BALB>129 (4/4) |
| SHIRPA | crossings | P<0.0001 | P<0.0001 | P<0.0001 | C57>C3H, BALB>129 (4/5) |
| Grip strength | 2-paw force | P<0.0001 | P<0.0001 | P=0.0025 | C57<129<C3H (5/5) |
| Grip strength | 4-paw force | P<0.0001 | P=0.039 | P=0.229 | C57<129<C3H (3/4) |
| Rotarod 1st validation | latency to fall | P=0.0003 | P<0.0001 | P<0.0001 | not consistent |
| Rotarod 2nd validation | latency to fall | P<0.0001 | P<0.0001 | P=0.0143 | C57>C3H>129 (3/3) |
| Y-maze | %SAP | P=0.0006 | P=0.962 | P=0.1114 | 129<C57, C3H, BALB (3/3) |
| Y-maze | tot entries | P<0.0001 | P<0.0001 | P=0.0003 | 129<C57, C3H, BALB (3/3) |
| ASR-PPI | startle response | P<0.0001 | P<0.0001 | P<0.0001 | C57<C3H, BALB, 129 (4/4) |
| ASR-PPI | % PPI | P<0.0001 | P<0.0001 | P=0.0037 | C57, BALB<C3H<129 (4/4) |
| Tail flick | latency | P=0.0014 | P<0.0001 | P=0.0006 | C3H>C57>129 (2/3) |
The table illustrates the level of significance of main effects (strain or center) and 2-way interaction (strain × center) from factorial ANOVA as well as providing a summary of the consistency of strain rankings across centers. SHIRPA, Smithkline Beecham-MRC Harwell-Imperial College-Royal London Hospital phenotype assessment; %SAP, percentage of spontaneous alternation performance.