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Ori4021 is active in the genome. (A) Sketch showing the migration patterns of various types of replication intermediates during neutral/neutral 2D gel electrophoresis. B) A restriction map of the genomic locus shows the two BamHI and BglII restriction fragments that are separated on a 2D gel and hybridized with an ori4021 probe. The pause signal observed along the Y arc on the BglII digest is not discussed here.
