Figure 4.

Mapping the minimal functional fragment of ori1068 (A) and ori3018 (B). Position and orientation of the synthetic primers are shown on the top line. Nucleotides positions are according to GenBank M91601 and M91600. The strain INAG3122 was transformed by electroporation with centromeric plasmids containing the reduced origins, because only replicative transformation is obtained at high frequency with this method. Results of transformation are shown by + and −. NR, Not recovered in E. coli.