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Double immunofluorescence microscopy of micronuclear mitotic apparatuses. Cells were permeabilized, fixed, and stained with anti-tubulin and anti-phosphorylated histone antibodies. The cells were viewed by confocal scanning laser fluorescence microscopy. The microtubules are green, the chromatin is red, and yellow is the superposition of the two stains. Top, wild-type micronuclear mitosis. Bottom, KO-1 micronuclear mitosis. Bar, 10 μm.
