Figure 2.

Ste2p localization is quantitatively lost, in a time- and α-factor-dependent manner from the cell surface of wild-type cells at 25 and 37°C. In the endocytosis mutant, sac6Δ Ste2p remains localized to the cell surface after exposure to α-factor at 25°C. To indicate nonspecific background, the average number of anti-Ste2p antibody localization to the cell surface of ste2Δ cells grown at 25°C is shown.