Figure 5.

Ste2p is nonrandomly distributed between invaginated and noninvaginated areas of the cell surface. Percentage of cell surface–associated Ste2p that is in furrow-like plasma membrane invaginations (PMIs) in wild-type cells exposed to α-factor at 25 or 37°C and sac6Δ cells at 25°C is plotted. A total of 50 individual cell sections at each time point were counted for each sample. Background localization to invaginations was 33 ± 2.4% (averaged over all points) of the total cell surface background localization; this was subtracted from all data points shown.