Figure 6.

Ste2p (yellow in merged image) is localized to vesicles that are proximal and perhaps continuous with furrow-like invaginations of the plasma membrane (arrows) and colocalizes with actin (red in merged image). Adjacent-face double localization of Ste2p and actin in wild-type cells before (A and B) and after (t = 5 min; C and D) exposure to α-factor is shown. Note that the anti-Ste2p section in A does not appear to be associated with a plasma membrane invagination until the adjacent section is examined. Note also in D that the “tip” of the furrow-like invagination (asterisk) has anti-actin antibodies localized around it. Bar, 0.1 μm.