Figure 7.

Antibodies directed against the vacuolar protease CPY (A) and the 90-kDa subunit of the vacuolar H⁺ ATPase Vph1p (B) localize to the vacuole and vacuole-associated compartments in wild-type cells. Note that the anti-CPY antibodies localize within the vacuole and the vacuole-associated compartment and that anti-Vph1p antibodies localize to the membrane of the vacuole and the perivacuole compartment. These perivacuole compartments, but not the vacuole, localize antibodies directed against Ypt51p (C and D). Note that the vacuole-associated compartments appear as tubular–vesicular structures having internal membranes. V, vacuole; g, Golgi. Bar, 0.5 μm.